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A Simple Key For Filling in Sterile Manufacturing Unveiled

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A seed-inventory method must be employed for lengthy-time period storage of cultures. Cultures obtained from your ATCC needs to be resuscitated Based on directions. If grown in broth, the cells are pelleted by centrifugation. Resuspend in 1/twentieth the quantity of clean routine maintenance broth, and increase an equal volume of twenty% (v/v in wa

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